With the active development of mobile devices, a variety of ultra-small, high-definition, and open platform-based cameras are being mass-produced.In this paper, we established an emulation system to verify the bio-imaging performance of the bulky and expensive high-performance cameras and various smartphone Fountains cameras that have been used in bio-imaging devices.In the proposed system, the linearity of the brightness gradient change of four types SHIRTS URBAN SHORT SLV of cameras was compared and analyzed.Based on these results, three cameras were selected in order of excellent linearity, and gel image analysis results were compared.